Organ Procurement
The organ procurement procedure is without a doubt one of the most important factors in attaining high quality therapeutic islets for clinical transplantation. Quickly cooled and procured organs handled carefully usually result in great isolations.
The procedure recommended for pancreas harvest uses en bloc removal of the pancreas in conjunction with liver procurement in order to quickly remove the organ. Subsequent back table dissection should use a hands-off-the-pancreas technique using the duodenum and spleen as handles. Keeping the organ cold throughout the entire procedure is of utmost importance.